Jahid Alakbarli

Bioprinting of Modified 3D Alginate-Based Glioblastoma Constructs via Combined Extrusion and Drop-on-Demand Approaches

 Supervisors: Dr. Rainer Detsch, Prof. Aldo R. Boccaccini

 3D tissue models are crucial for a better understanding of cancer biology and evaluating therapeutic strategies. The generation of three-dimensional tumor models is a promising approach that has the potential to resemble the complex architecture of tumors [1]. Alginate-based bioinks have demonstrated significant potential for replicating tumor-like mechanical and biochemical environments, supporting cell viability, and enabling the construction of physiologically relevant cancer models [2]. This project focuses on developing a 3D bioprinted alginate-based glioblastoma model by integrating extrusion and Drop-on-Demand (DoD) bioprinting techniques [3]. The 3D alginate-based constructs are evaluated through physicochemical and biological analyses, contributing to the advancement of tissue engineering and cancer research.

[1]      [1] R. E. Horch et al., “Cancer research by means of tissue engineering – is there a rationale?,” J Cell Mol Med, vol. 17, no. 10, p. 1197, Oct. 2013.

[2]      [2] V. Bednarzig et al., “Advanced ADA-GEL bioink for bioprinted artificial cancer models,” Bioprinting, vol. 23, p. e00145, Aug. 2021.

[3]      [3] E. Karakaya et al., “Engineering peptide-modified alginate-based bioinks with cell-adhesive properties for biofabrication,” RSC Adv, vol. 14, no. 20, pp. 13769–13786, Apr. 2024.