3D-printing of pre-crosslinked alginate hydrogel scaffolds for bone defects
Supervisors: Vera Bednarzig, Prof. Aldo R. Boccaccini
Over the last decades 3D-printing has become increasingly important for tissue engineering. The technique offers the opportunity to create scaffolds and implants individually for every patient. A frequently used material is the natural polymer alginate [1, 2]. It can be combined with calcium-carbonate (CaCO3) and other components to form a pre-crosslinked gel . This hydrogel shows good degradability, ease of processing and the ability to immobilize incorporated cells. A secondary (final) crosslinking process, by adding CaCl2 or BaCl2 is necessary, to increase the mechanical properties and to form a stable gel. The aim of this study is to create a multilayer scaffold with the pre-crosslinked hydrogel alginate printed on top of a metallic mesh and to characterize the mechanical properties of the new construct and the connection between metal substrate and hydrogel.
 B. Sarker et al., Fabrication of alginate–gelatin crosslinked hydrogel microcapsules and evaluation of the microstructure and physico-chemical properties, Journal of Materials Chemistry B 2 (2014) 1470-1482.
 L. Wang, R.M. Shelton, P.R. Cooper, M. Lawson, J.T. Triffitt, J.E. Barralet. Evaluation of sodium alginate for bone marrow cell tissue engineering. Biomaterials 24 (2003) 3475–3481